The extraction of Peste Des Petits Ruminants Virus RNA from paraffin-embedded tissues using a modified extraction method

Peste des petits ruminants (PPR) which is caused by small ruminant morbillivirus (PPRV) has an important economic impact on farming due to high mortality rates, weight loss and restrictions the export of products. Molecular assays are commonly used in diagnosis disease. Extraction RNA from formalin-fixed paraffin-embedded (FFPE) tissues challenging because often degraded formalin fixation process. Although commercial kits have been developed for extraction nucleic acids FFPE tissues, they expensive than other kits. In this study, a modified method was evaluated detection PPRV tissues. A total 20 tissue samples including 15 positive 5 negative were used. Two years ago, these selected analysed nucleoprotein gene based one step real time RT-PCR before fixed with embedded paraffin. extracted using tested fusion (F) RT-PCR. specific detected 12 whereas 3 found one-step Furthermore, also negative. Three false results real-time cycle threshold. Therefore, could be related lower viral loads might limit The study show that had stored 2 years.